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Transcript residency on ribosomes reveals a key role for the A rabidopsis thaliana bundle sheath in sulfur and glucosinolate metabolism
Author(s) -
Aubry Sylvain,
SmithUnna Richard D.,
Boursnell Chris M.,
Kopriva Stanislav,
Hibberd Julian M.
Publication year - 2014
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12502
Subject(s) - biology , phloem , arabidopsis thaliana , vascular bundle , microbiology and biotechnology , ribosome , biochemistry , glucosinolate , arabidopsis , gene , botany , rna , mutant , brassica
Summary Leaves of angiosperms are made up of multiple distinct cell types. While the function of mesophyll cells, guard cells, phloem companion cells and sieve elements are clearly described, this is not the case for the bundle sheath ( BS ). To provide insight into the role of the BS in the C 3 species A rabidopsis thaliana , we labelled ribosomes in this cell type with a FLAG tag. We then used immunocapture to isolate these ribosomes, followed by sequencing of resident m RNA s. This showed that 5% of genes showed specific splice forms in the BS , and that 15% of genes were preferentially expressed in these cells. The BS translatome strongly implies that the BS plays specific roles in sulfur transport and metabolism, glucosinolate biosynthesis and trehalose metabolism. Much of the C 4 cycle is differentially expressed between the C 3 BS and the rest of the leaf. Furthermore, the global patterns of transcript residency on BS ribosomes overlap to a greater extent with cells of the root pericycle than any other cell type. This analysis provides the first insight into the molecular function of this cell type in C 3 species, and also identifies characteristics of BS cells that are probably ancestral to both C 3 and C 4 plants.