Premium
TaADF7, an actin‐depolymerizing factor, contributes to wheat resistance against Puccinia striiformis f. sp. tritici
Author(s) -
Fu Yanping,
Duan Xiaoyuan,
Tang Chunlei,
Li Xiaorui,
Voegele Ralf T.,
Wang Xiaojie,
Wei Guorong,
Kang Zhensheng
Publication year - 2014
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12457
Subject(s) - biology , cytoskeleton , actin , microbiology and biotechnology , cytochalasin d , actin cytoskeleton , genetics , cell
Summary The actin cytoskeleton is involved in plant defense responses; however, the role of the actin‐depolymerizing factor (ADF) family, which regulates actin cytoskeletal dynamics, in plant disease resistance, is largely unknown. Here, we characterized a wheat ( Triticum aestivum ) ADF gene, TaADF7 , with three copies located on chromosomes 1A, 1B, and 1D, respectively. All three copies encoded the same protein, although there were variations in 19 nucleotide positions in the open reading frame. Transcriptional expression of the three TaADF7 copies were all sharply elevated in response to avirulent Puccinia striiformis f. sp. tritici ( Pst ) infection, with similar expression patterns. TaADF7 regulated the actin cytoskeletal dynamics by targeting the actin cytoskeleton to execute actin binding/severing activities. When the TaADF7 copies were all silenced by virus‐induced gene silencing, the growth of Pst hypha increased and sporadic urediniospores were observed, as compared with control plants, upon inoculation with avirulent Pst . In addition, the accumulation of reactive oxygen species (ROS) and the hypersensitive response (HR) were greatly weakened, whereas cytochalasin B partially rescued the HR in TaADF7 knock‐down plants. Together, these findings suggest that TaADF7 is likely to contribute to wheat resistance against Pst infection by modulating the actin cytoskeletal dynamics to influence ROS accumulation and the HR.