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The TRANSPLANTA collection of A rabidopsis lines: a resource for functional analysis of transcription factors based on their conditional overexpression
Author(s) -
Coego Alberto,
Brizuela Esther,
Castillejo Pablo,
Ruíz Sandra,
Koncz Csaba,
del Pozo Juan C.,
Piñeiro Manuel,
Jarillo José A.,
PazAres Javier,
León José
Publication year - 2014
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12443
Subject(s) - biology , transcription factor , arabidopsis , gene , regulator , computational biology , genetics , microbiology and biotechnology , phenotype , mutant
Summary Transcription factors ( TF s) are key regulators of gene expression in all organisms. In eukaryotes, TF s are often represented by functionally redundant members of large gene families. Overexpression might prove a means to unveil the biological functions of redundant TF s; however, constitutive overexpression of TF s frequently causes severe developmental defects, preventing their functional characterization. Conditional overexpression strategies help to overcome this problem. Here, we report on the T RANSPLANTA collection of Arabidopsis lines, each expressing one of 949 TF s under the control of a β–estradiol‐inducible promoter. Thus far, 1636 independent homozygous lines, representing an average of 2.6 lines for every TF , have been produced for the inducible expression of 634 TF s. Along with a GUS ‐ GFP reporter, randomly selected TRANSPLANTA lines were tested and confirmed for conditional transgene expression upon β–estradiol treatment. As a proof of concept for the exploitation of this resource, β–estradiol‐induced proliferation of root hairs, dark‐induced senescence, anthocyanin accumulation and dwarfism were observed in lines conditionally expressing full‐length cDNA s encoding RHD 6, WRKY 22, MYB 123/ TT 2 and MYB 26, respectively, in agreement with previously reported phenotypes conferred by these TF s. Further screening performed with other TRANSPLANTA lines allowed the identification of TF s involved in different plant biological processes, illustrating that the collection is a powerful resource for the functional characterization of TF s. For instance, ANAC 058 and a TINY / AP 2 TF were identified as modulators of ABA ‐mediated germination potential, and RAP 2.10/ DEAR 4 was identified as a regulator of cell death in the hypocotyl–root transition zone. Seeds of TRANSPLANTA lines have been deposited at the Nottingham Arabidopsis Stock Centre for further distribution.