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The Arabidopsis Mediator subunit MED 16 regulates iron homeostasis by associating with EIN 3/ EIL 1 through subunit MED 25
Author(s) -
Yang Yan,
Ou Bin,
Zhang Jinzhe,
Si Wen,
Gu Hongya,
Qin Genji,
Qu LiJia
Publication year - 2014
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12440
Subject(s) - arabidopsis , microbiology and biotechnology , transcription factor , homeostasis , mutant , protein subunit , iron homeostasis , mediator , chemistry , downregulation and upregulation , biology , biochemistry , gene
Summary Iron is an essential micronutrient for plants and animals, and plants are a major source of iron for humans. Therefore, understanding the regulation of iron homeostasis in plants is critical. We identified a T‐ DNA insertion mutant, yellow and sensitive to iron‐deficiency 1 ( yid1 ), that was hypersensitive to iron deficiency, containing a reduced amount of iron. YID 1 encodes the Arabidopsis Mediator complex subunit MED 16. We demonstrated that YID 1/ MED 16 interacted with another subunit, MED 25. MED 25 played an important role in regulation of iron homeostasis by interacting with EIN 3 and EIL 1, two transcription factors in ethylene signaling associated with regulation of iron homeostasis. We found that the transcriptome in yid1 and med25 mutants was significantly affected by iron deficiency. In particular, the transcription levels of FIT , IRT 1 and FRO 2 were reduced in the yid1 and med25 mutants under iron‐deficient conditions. The finding that YID 1/ MED 16 and MED 25 positively regulate iron homeostasis in Arabidopsis increases our understanding of the complex transcriptional regulation of iron homeostasis in plants.