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ABCG 9, ABCG 11 and ABCG 14 ABC transporters are required for vascular development in A rabidopsis
Author(s) -
Hir Rozenn,
Sorin Clément,
Chakraborti Dipankar,
Moritz Thomas,
Schaller Hubert,
Tellier Frédérique,
Robert Stéphanie,
Morin Halima,
Bako Laszlo,
Bellini Catherine
Publication year - 2013
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12334
Subject(s) - atp binding cassette transporter , biology , microbiology and biotechnology , chemistry , biochemistry , transporter , genetics , gene
Summary In order to obtain insights into the regulatory pathways controlling phloem development, we characterized three genes encoding membrane proteins from the G sub‐family of ABC transporters ( ABCG 9 , ABCG 11 and ABCG 14 ), whose expression in the phloem has been confirmed. Mutations in the genes encoding these dimerizing ‘half transporters’ are semi‐dominant and result in vascular patterning defects in cotyledons and the floral stem. Co‐immunoprecipitation and bimolecular fluorescence complementation experiments demonstrated that these proteins dimerize, either by flexible pairing ( ABCG 11 and ABCG 9) or by forming strict heterodimers ( ABCG 14). In addition, metabolome analyses and measurement of sterol ester contents in the mutants suggested that ABCG 9, ABCG 11 and ABCG 14 are involved in lipid/sterol homeostasis regulation. Our results show that these three ABCG genes are required for proper vascular development in A rabidopsis thaliana .