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Apical F ‐actin‐regulated exocytic targeting of N t PPME 1 is essential for construction and rigidity of the pollen tube cell wall
Author(s) -
Wang Hao,
Zhuang Xiaohong,
Cai Yi,
Cheung Alice Y.,
Jiang Liwen
Publication year - 2013
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12300
Subject(s) - pollen tube , microbiology and biotechnology , exocytosis , tip growth , actin , actin cytoskeleton , cytoskeleton , biology , biophysics , pollen , cell , secretion , biochemistry , botany , pollination
Summary In tip‐confined growing pollen tubes, delivery of newly synthesized cell wall materials to the rapidly expanding apical surface requires spatial organization and temporal regulation of the apical F ‐actin filament and exocytosis. In this study, we demonstrate that apical F ‐actin is essential for the rigidity and construction of the pollen tube cell wall by regulating exocytosis of N icotiana tabacum pectin methylesterase ( N t PPME 1). Wortmannin disrupts the spatial organization of apical F ‐actin in the pollen tube tip and inhibits polar targeting of N t PPME 1, which subsequently alters the rigidity and pectic composition of the pollen tube cell wall, finally causing growth arrest of the pollen tube. In addition to mechanistically linking cell wall construction and apical F ‐actin, wortmannin can be used as a useful tool for studying endomembrane trafficking and cytoskeletal organization in pollen tubes.