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Blue‐light‐induced PIN 3 polarization for root negative phototropic response in Arabidopsis
Author(s) -
Zhang KunXiao,
Xu HengHao,
Yuan TingTing,
Zhang Liang,
Lu YingTang
Publication year - 2013
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12298
Subject(s) - phototropism , auxin , arabidopsis , gravitropism , brefeldin a , phototropin , biophysics , blue light , microbiology and biotechnology , chemistry , biology , biochemistry , physics , optics , gene , mutant , golgi apparatus , endoplasmic reticulum
Summary Root negative phototropism is an important response in plants. Although blue light is known to mediate this response, the cellular and molecular mechanisms underlying root negative phototropism remain unclear. Here, we report that the auxin efflux carrier PIN ‐ FORMED ( PIN ) 3 is involved in asymmetric auxin distribution and root negative phototropism. Unilateral blue‐light illumination polarized PIN 3 to the outer lateral membrane of columella cells at the illuminated root side, and increased auxin activity at the illuminated side of roots, where auxin promotes growth and causes roots bending away from the light source. Furthermore, root negative phototropic response and blue‐light‐induced PIN 3 polarization were modulated by a brefeldin A‐sensitive, GNOM ‐dependent, trafficking pathway and by phot1‐regulated PINOID ( PID )/ PROTEIN PHOSPHATASE 2A ( PP 2A) activity. Our results indicate that blue‐light‐induced PIN 3 polarization is needed for asymmetric auxin distribution during root negative phototropic response.