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Identification and characterization of functional centromeres of the common bean
Author(s) -
Iwata Aiko,
Tek Ahmet L.,
Richard Ma M.S.,
Abernathy Brian,
Fonsêca Artur,
Schmutz Jeremy,
Chen Nicolas W.G.,
Thareau Vincent,
Magdelenat Ghislaine,
Li Yupeng,
Murata Minoru,
PedrosaHarand Andrea,
Geffroy Valérie,
Nagaki Kiyotaka,
Jackson Scott A.
Publication year - 2013
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12269
Subject(s) - centromere , biology , satellite dna , genome , genetics , chromosome , chromatin immunoprecipitation , fluorescence in situ hybridization , evolutionary biology , gene , gene expression , promoter
Summary In higher eukaryotes, centromeres are typically composed of megabase‐sized arrays of satellite repeats that evolve rapidly and homogenize within a species' genome. Despite the importance of centromeres, our knowledge is limited to a few model species. We conducted a comprehensive analysis of common bean ( P haseolus vulgaris ) centromeric satellite DNA using genomic data, fluorescence in situ hybridization ( FISH ), immunofluorescence and chromatin immunoprecipitation (Ch IP ). Two unrelated centromere‐specific satellite repeats, C ent P v1 and C ent P v2, and the common bean centromere‐specific histone H 3 ( P v CENH 3) were identified. FISH showed that C ent P v1 and C ent P v2 are predominantly located at subsets of eight and three centromeres, respectively. Immunofluorescence‐ and Ch IP ‐based assays demonstrated the functional significance of C ent P v1 and C ent P v2 at centromeres. Genomic analysis revealed several interesting features of C ent P v1 and C ent P v2: (i) C ent P v1 is organized into an higher‐order repeat structure, named N azca, of 528 bp, whereas C ent P v2 is composed of tandemly organized monomers; (ii) C ent P v1 and C ent P v2 have undergone chromosome‐specific homogenization; and (iii) C ent P v1 and C ent P v2 are not likely to be commingled in the genome. These findings suggest that two distinct sets of centromere sequences have evolved independently within the common bean genome, and provide insight into centromere satellite evolution.