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MIDGET connects COP 1‐dependent development with endoreduplication in A rabidopsis thaliana
Author(s) -
Schrader Andrea,
Welter Bastian,
Hulskamp Martin,
Hoecker Ute,
Uhrig Joachim F.
Publication year - 2013
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12199
Subject(s) - endoreduplication , biology , microbiology and biotechnology , arabidopsis thaliana , photomorphogenesis , genetics , mutant , arabidopsis , cell , gene , cell cycle
Summary In A rabidopsis thaliana , loss of CONSTITUTIVE PHOTOMORPHOGENIC 1 ( COP 1) function leads to constitutive photomorphogenesis in the dark associated with inhibition of endoreduplication in the hypocotyl, and a post‐germination growth arrest. MIDGET ( MID ), a component of the TOPOISOMERASE VI ( TOPOVI ) complex, is essential for endoreduplication and genome integrity in A . thaliana . Here we show that MID and COP 1 interact in vitro and in vivo through the amino terminus of COP 1. We further demonstrate that MID supports sub‐nuclear accumulation of COP 1. The MID protein is not degraded in a COP 1‐dependent fashion in darkness, and the phenotypes of single and double mutants prove that MID is not a target of COP 1 but rather a necessary factor for proper COP 1 activity with respect to both, control of COP 1‐dependent morphogenesis and regulation of endoreduplication. Our data provide evidence for a functional connection between COP 1 and the TOPOVI in plants linking COP 1‐dependent development with the regulation of endoreduplication.