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An Arabidopsis ATPase gene involved in nematode‐induced syncytium development and abiotic stress responses
Author(s) -
Ali Muhammad Amjad,
Plattner Stephan,
Radakovic Zoran,
Wieczorek Krzysztof,
Elashry Abdelnaser,
Grundler Florian M.W.,
Ammelburg Moritz,
Siddique Shahid,
Bohlmann Holger
Publication year - 2013
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12170
Subject(s) - arabidopsis , syncytium , nematode , microbiology and biotechnology , gene , biology , abiotic component , abiotic stress , atpase , botany , genetics , ecology , biochemistry , mutant , cell , enzyme
Summary The beet cyst nematode H eterodera schachtii induces syncytia in the roots of A rabidopsis thaliana , which are its only nutrient source. One gene, At1g64110 , that is strongly up‐regulated in syncytia as shown by RT ‐ PCR , quantitative RT ‐ PCR , in situ RT ‐ PCR and promoter:: GUS lines, encodes an AAA +‐type ATP ase. Expression of two related genes in syncytia, At4g28000 and At5g52882 , was not detected or not different from control root segments. Using ami RNA lines and T‐ DNA mutants, we show that At1g64110 is important for syncytium and nematode development. At1g64110 was also inducible by wounding, jasmonic acid, salicylic acid, heat and cold, as well as drought, sodium chloride, abscisic acid and mannitol, indicating involvement of this gene in abiotic stress responses. We confirmed this using two T ‐ DNA mutants that were more sensitive to abscisic acid and sodium chloride during seed germination and root growth. These mutants also developed significantly smaller roots in response to abscisic acid and sodium chloride. An in silico analysis showed that ATP ase A t1g64110 (and also A t4g28000 and A t5g52882) belong to the ‘meiotic clade’ of AAA proteins that includes proteins such as V ps4, k atanin, s pastin and MSP 1.