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B rassica napus TT16 homologs with different genomic origins and expression levels encode proteins that regulate a broad range of endothelium‐associated genes at the transcriptional level
Author(s) -
Chen Guanqun,
Deng Wei,
Peng Fred,
Truksa Martin,
Singer Stacy,
Snyder Crystal L.,
Mietkiewska Elzbieta,
Weselake Randall J.
Publication year - 2013
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12151
Subject(s) - biology , gene , transcription factor , function (biology) , rna interference , microbiology and biotechnology , genetics , gene expression , rna
Summary The transcription factor TRANSPARENT TESTA 16 ( TT 16) plays an important role in endothelial cell specification and proanthocyanidin ( PA ) accumulation. However, its precise regulatory function with regard to the expression of endothelial‐associated genes in developing seeds, and especially in the PA ‐producing inner integument, remains largely unknown. Therefore, we endeavored to characterize four TT16 homologs from the allotetraploid oil crop species B rassica napus , and systematically explore their regulatory function in endothelial development. Our results indicated that all four BnTT16 genes were predominantly expressed in the early stages of seed development, but at distinct levels, and encoded functional proteins. Bntt16 RNA interference lines exhibited abnormal endothelial development and decreased PA content, while PA polymerization was not affected. In addition to the previously reported function of TT 16 in the transcriptional regulation of anthocyanidin reductase ( ANR ) and dihydroflavonol reductase ( TT3 ), we also determined that BnTT16 proteins played a significant role in the transcriptional regulation of five other genes involved in the PA biosynthetic pathway ( P < 0.01). Moreover, we identified two genes involved in inner integument development that were strongly regulated by the BnTT16 proteins ( TT2 and δ‐vacuolar processing enzyme ). These results will better our understanding of the precise role of TT16 in endothelial development in B rassicaceae species, and could potentially be used for the future improvement of oilseed crops.