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A RING ‐type E 3 ligase controls anther dehiscence by activating the jasmonate biosynthetic pathway gene DEFECTIVE IN ANTHER DEHISCENCE 1 in A rabidopsis
Author(s) -
Peng YanJhu,
Shih ChingFang,
Yang JunYi,
Tan ChoonMeng,
Hsu WeiHan,
Huang YuPei,
Liao PeiChun,
Yang ChangHsien
Publication year - 2013
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12122
Subject(s) - ubiquitin ligase , biology , jasmonic acid , arabidopsis , mutant , ectopic expression , microbiology and biotechnology , tapetum , stamen , gene , genetics , ubiquitin , botany , pollen , microspore
Summary Suppression of expression of DAF [ DEFECTIVE IN ANTHER DEHISCENCE 1 ( DAD 1 )‐ Activating Factor ], a gene that encodes a putative RING ‐finger E3 ligase protein, causes non‐dehiscence of the anthers, alters pollen development and causes sterility in 35S: DAF RNA i/antisense Arabidopsis plants. This mutant phenotype correlates with the suppression of DAF but not with expression of the two most closely related genes, DAFL 1 / 2 . The expression of DAD 1 was significantly reduced in 35S: DAF RNA i/antisense plants, and complementation with 35S: DAF did not rescue the dad1 mutant, indicating that DAF acts upstream of DAD 1 in jasmonic acid biosynthesis. This assumption is supported by the finding that 35S: DAF RNA i/antisense plants showed a similar cellular basis for anther dehiscence to that found in dad1 mutants, and that external application of jasmonic acid rescued the anther non‐dehiscence and pollen defects in 35S: DAF antisense flowers. We further demonstrate that DAF is an E3 ubiquitin ligase and that its activity is abolished by C132S and H137Y mutations in its RING motif. Furthermore, ectopic expression of the dominant‐negative C 132 S or H 137 Y mutations causes similar indehiscence of anthers and reduction in DAD 1 expression in transgenic Arabidopsis. This result not only confirms that DAF controls anther dehiscence by positively regulating the expression of DAD 1 in the jasmonic acid biosynthesis pathway, but also supports the notion that DAF functions as an E 3 ubiquitin ligase, and that the conserved RING ‐finger region is required for its activity.