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Vascular plant one‐zinc‐finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Author(s) -
Nakai Yusuke,
Nakahira Yoichi,
Sumida Hiroki,
Takebayashi Kosuke,
Nagasawa Yumiko,
Yamasaki Kanako,
Akiyama Masako,
OhmeTakagi Masaru,
Fujiwara Sumire,
Shiina Takashi,
Mitsuda Nobutaka,
Fukusaki Eiichiro,
Kubo Yasuyuki,
Sato Masa H.
Publication year - 2013
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12069
Subject(s) - abscisic acid , jasmonic acid , abiotic stress , biotic stress , microbiology and biotechnology , salicylic acid , transcription factor , biology , abiotic component , proteasome , zinc finger , arabidopsis , mutant , biochemistry , gene , ecology
Summary Plants adapt to abiotic and biotic stresses by activating abscisic acid‐mediated (ABA) abiotic stress‐responsive and salicylic acid‐(SA) or jasmonic acid‐mediated (JA) biotic stress‐responsive pathways, respectively. Although the abiotic stress‐responsive pathway interacts antagonistically with the biotic stress‐responsive pathways, the mechanisms that regulate these pathways remain largely unknown. In this study, we provide insight into the function of vascular plant one‐zinc‐finger proteins ( VOZ s) that modulate various stress responses in A rabidopsis. The expression of many stress‐responsive genes was changed in the voz1voz2 double mutant under normal growth conditions. Consistent with altered stress‐responsive gene expression, freezing‐ and drought‐stress tolerances were increased in the voz1voz2 double mutant. In contrast, resistance to a fungal pathogen, C olletotrichum higginsianum , and to a bacterial pathogen, P seudomonas syringae , was severely impaired. Thus, impairing VOZ function simultaneously conferred increased abiotic tolerance and biotic stress susceptibility. In a chilling stress condition, both the VOZ1 and VOZ2 m RNA expression levels and the VOZ 2 protein level gradually decreased. VOZ 2 degradation during cold exposure was completely inhibited by the addition of the 26 S proteasome inhibitor, MG 132, a finding that suggested that VOZ 2 degradation is dependent on the ubiquitin/26 S proteasome system. In voz1voz2 , ABA ‐inducible transcription factor CBF 4 expression was enhanced significantly even under normal growth conditions, despite an unchanged endogenous ABA content. A finding that suggested that VOZ s negatively affect CBF4 expression in an ABA ‐independent manner. These results suggest that VOZ s function as both negative and positive regulators of the abiotic and biotic stress‐responsive pathways, and control Arabidopsis adaptation to various stress conditions.