Premium
Calmodulin‐related CML 24 interacts with ATG 4b and affects autophagy progression in A rabidopsis
Author(s) -
Tsai YuChang,
Koo Yeonjong,
Delk Nikkí A.,
Gehl Bernadette,
Braam Janet
Publication year - 2013
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12043
Subject(s) - autophagy , arabidopsis , microbiology and biotechnology , calmodulin , biology , mutant , saccharomyces cerevisiae , in vitro , yeast , genetics , biochemistry , gene , apoptosis , enzyme
Summary Plants encounter environmental stress challenges that are distinct from those of other eukaryotes because of their relative immobility. Therefore, plants may have evolved distinct regulatory mechanisms for conserved cellular functions. Plants, like other eukaryotes, share aspects of both calcium‐ and calmodulin‐based cellular signaling and the autophagic process of cellular renewal. Here, we report a novel function for an A rabidopsis calmodulin‐related protein, CML 24, and insight into ATG 4‐regulated autophagy. CML 24 interacts with ATG 4b in yeast two‐hybrid, in vitro pull‐down and transient tobacco cell transformation assays. Mutants with missense mutations in CML 24 have aberrant ATG 4 activity patterns in in vitro extract assays, altered ATG 8 accumulation levels, an altered pattern of GFP – ATG 8‐decorated cellular structures, and altered recovery from darkness‐induced starvation. Together, these results support the conclusion that CML 24 affects autophagy progression through interactions with ATG 4.