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ZmGA3ox2 , a candidate gene for a major QTL , qPH3.1 , for plant height in maize
Author(s) -
Teng Feng,
Zhai Lihong,
Liu Ruixiang,
Bai Wei,
Wang Liqiu,
Huo Dongao,
Tao Yongsheng,
Zheng Yonglian,
Zhang Zuxin
Publication year - 2013
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12038
Subject(s) - quantitative trait locus , biology , plant stem , candidate gene , locus (genetics) , gene , genetics , mutant , chromosome , doubled haploidy , horticulture , agronomy
Summary Maize plant height is closely associated with biomass, lodging resistance and grain yield. Determining the genetic basis of plant height by characterizing and cloning plant height genes will guide the genetic improvement of crops. In this study, a quantitative trait locus ( QTL ) for plant height, qPH3.1 , was identified on chromosome 3 using populations derived from a cross between Z ong3 and its chromosome segment substitution line, SL 15. The plant height of the two lines was obviously different, and application of exogenous gibberellin A 3 removed this difference. QTL mapping placed qPH3.1 within a 4.0 cM interval, explaining 32.3% of the phenotypic variance. Furthermore, eight homozygous segmental isolines ( SIL s) developed from two larger F 2 populations further narrowed down qPH3.1 to within a 12.6 kb interval. ZmGA3ox2 , an ortholog of OsGA3ox2 , which encodes a GA3 β ‐ hydroxylase, was positionally cloned. Association mapping identified two polymorphisms in ZmGA3ox2 that were significantly associated with plant height across two experiments. Quantitative RT ‐ PCR showed that SL 15 had higher ZmGA3ox2 expression relative to Z ong3. The resultant higher GA 1 accumulation led to longer internodes in SL 15 because of increased cell lengths. Moreover, a large deletion in the coding region of ZmGA3ox2 is responsible for the dwarf mutant d1‐6016 . The successfully isolated qPH3.1 enriches our knowledge on the genetic basis of plant height in maize, and provides an opportunity for improvement of plant architecture in maize breeding.