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BAT 1, a putative acyltransferase, modulates brassinosteroid levels in A rabidopsis
Author(s) -
Choi Sunhwa,
Cho Younghyun,
Kim Kangmin,
Matsui Minami,
Son SeungHyun,
Kim SeongKi,
Fujioka Shozo,
Hwang Ildoo
Publication year - 2013
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12036
Subject(s) - brassinolide , chemistry , brassinosteroid , endoplasmic reticulum , acyltransferase , biochemistry , antioxidant , microbiology and biotechnology , arabidopsis , enzyme , gene , botany , mutant , plant growth , biology
Summary Brassinosteroids ( BR s) are essential for various aspects of plant development. Cellular BR homeostasis is critical for proper growth and development of plants; however, its regulatory mechanism remains largely unknown. BAT1 ( BR ‐related acyltransferase 1 ), a gene encoding a putative acyltransferase, was found to be involved in vascular bundle development in a full‐length cDNA over‐expressor ( FOX ) screen. Over‐expression of BAT 1 resulted in typical BR ‐deficient phenotypes, which were rescued by exogenously applied castasterone and brassinolide. Analyses of BR profiles demonstrated that BAT 1 alters levels of several brassinolide biosynthetic intermediates, including 6‐deoxotyphasterol, typhasterol and 6‐deoxocastasterone. BAT1 is mainly localized in the endoplasmic reticulum. BAT 1 is highly expressed in young tissues and vascular bundles, and its expression is induced by auxin. These data suggest that BAT 1 is involved in BR homeostasis, probably by conversion of brassinolide intermediates into acylated BR conjugates.