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Dengue in Santander State, Colombia: fluctuations in the prevalence of virus serotypes are linked to dengue incidence and genetic diversity of the circulating viruses
Author(s) -
Carreño María Fernanda,
Jiménez-Silva Cinthy Lorena,
ReyCaro Luz Aida,
CondeOcazionez Sergio Andrés,
Flechas-Alarcón María Camila,
Velandia Sindi Alejandra,
Ocazionez Raquel Elvira
Publication year - 2019
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/tmi.13311
Subject(s) - dengue fever , serotype , dengue virus , virology , genetic diversity , incidence (geometry) , biology , diversity (politics) , virus , geography , population , environmental health , medicine , physics , sociology , anthropology , optics
Objective To investigate the link between fluctuations in the prevalence of dengue virus (DENV) serotypes and the number of dengue cases in the metropolitan area of Bucaramanga, Santander State, Colombia, in the 2007–2010 and 2014–2017 periods. Method Viruses were isolated from febrile patient samples by direct application to C6/36‐HT cells and typed using monoclonal antibodies. We performed autocorrelation and cross‐correlation analyses to determine whether fluctuations in the prevalence of DENV serotypes and dengue cases were correlated. Full envelope (E) gene sequences were employed to examine the genetic diversity of serotypes circulating by using a phylogenetic approach. Results All four dengue virus serotypes were detected. DENV‐1 was the dominant serotype in both periods followed by DENV‐3 or DENV‐2 depending on the period; DENV‐4 was the least prevalent virus in both periods. Cross‐correlation analyses suggest a temporal relation between the fluctuations in the prevalence of DENV serotypes, which were almost simultaneous (lag = 0) or related to recent past fluctuations (lag > 1.0) in the number of dengue cases. Data suggest that a sustained predominance of DENV‐1, an increase of the DENV‐4 prevalence, and a switch from DENV‐3 to DENV‐2 could be linked to an outbreak. Circulating viruses were grouped into Genotype V, Asia/American III and II for DENV‐1, ‐2, ‐3 and ‐4, respectively; intragenotypic diversity was detected. Conclusions The present work highlights the need of comprehensive studies on dynamics of DENV in Colombia to understand transmission of dengue and evaluate the effectiveness of a vaccination programme.