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Vertical transmission of dengue virus in Aedes aegypti and its role in the epidemiological persistence of dengue in Central and Southern Mexico
Author(s) -
DanisLozano Rogelio,
DíazGonzález Esteban E.,
MaloGarcía Iliana R.,
Rodríguez Mario Henry,
RamosCastañeda José,
JuárezPalma Lilia,
Ramos Celso,
LópezOrdóñez Teresa,
MossoGonzález Clemente,
FernándezSalas Ildefonso
Publication year - 2019
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/tmi.13306
Subject(s) - aedes aegypti , dengue fever , dengue virus , aedes albopictus , biology , arbovirus , virology , transmission (telecommunications) , flavivirus , aedes , virus , ecology , larva , electrical engineering , engineering
Background Aedes aegypti and Aedes albopictus are the main mosquito species responsible for dengue virus ( DENV ) transmission to humans in the tropical and subtropical regions of the world. The role of vertical transmission in the epidemiology of dengue and the maintenance of this arbovirus in nature during interepidemic periods remain poorly understood, and DENV vertical transmission could sustain the existence of virus reservoirs within Aedes populations. Methods Between April 2011 and October 2012, we monitored vertical transmission of DENV in Ae. aegypti and Ae. albopictus in 9 cities of 4 Mexican states. Aedes eggs were collected in ovitraps, then adults were reared under laboratory conditions and their heads were used to infect C6/36 cells. The presence of flavivirus was detected by immunofluorescence assays ( IFA ), and DENV infection was confirmed by RT ‐ PCR . Results About 96% of reared adults were Ae. aegypti and 4.0% were Ae. albopictus . No infection was detected in Ae. albopictus , whereas 54 of 713 (7.8%) of Ae. aegypti pools tested positive. A minimum infection rate ( MIR ) of 2.52 per 1000 mosquitoes was estimated for Ae. aegypti . DENV ‐1, DENV ‐2 & DENV ‐3 serotypes were detected even during interepidemic periods. Conclusions This study reports the evidence of vertical transmission of dengue virus with viral isolation and molecular confirmation in Ae. aegypti eggs collected in four endemic regions of Central and Southern Mexico. Vertical transmission may play a role as a reservoir mechanism during mosquito dormancy in interepidemic periods but with minor participation in transmission during epidemic periods.