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Molecular epidemiology of methicillin‐resistant Staphylococcus aureus in the Brazilian primary health care system
Author(s) -
PereiraFranchi Eliane Patricia Lino,
Barreira Maria Rachel Nogueira,
Costa Natália de Sousa Lima Moreira,
Riboli Danilo Flávio Moraes,
Abraão Ligia Maria,
Martins Katheryne Benini,
Victória Cassiano,
Cunha Maria de Lourdes Ribeiro de Souza da
Publication year - 2019
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/tmi.13192
Subject(s) - pulsed field gel electrophoresis , multilocus sequence typing , microbiology and biotechnology , staphylococcus aureus , molecular epidemiology , leukocidin , typing , virulence , biology , methicillin resistant staphylococcus aureus , hemolysin , virology , genotype , gene , genetics , bacteria
Objective To evaluate the molecular epidemiology and to georeference Staphylococcus aureus isolated from wounds and nares of patients seen at Basic Health Units ( BHU s) of a Brazilian city. Methods Observational, cross‐sectional study conducted from 2010 to 2013. A total of 119 S. aureus strains isolated from the wounds and nares of 88 patients were studied. The isolates were characterised by identifying virulence genes encoding enterotoxins A–E, haemolysins α , β and δ , exfoliatins A, B and D, biofilm production, Panton‐Valentine Leukocidin and toxic shock syndrome toxin 1, and by pulsed‐field gel electrophoresis ( PFGE ), multilocus sequence and spa typing. Results Eighteen methicillin‐resistant Staphylococcus aureus (MRSA) (6 SCC mec type II and 12 SCC mec type IV ) and 101 (85%) M SSA were identified. PFGE typing resulted in the formation of eight clusters, with ST s 1, 5, 8, 30, 188, 1176 and 1635 and spa type t002 being the predominant types among MSSA . The 18 MRSA belonged to ST s 5, 8 and 1176 and spa types t002 and t062. Conclusion The results demonstrate widespread dissemination of MSSA and MRSA clones carrying haemolysin, biofilm and toxin genes. Kernel density estimation revealed the highest density of S. aureus in the 4, 5 and 8 BHU s.