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Seroprevalence of sarcocystosis in the local communities of Pangkor and Tioman Islands using recombinant surface antigens 3 ( rSAG 3) of Sarcocystis falcatula
Author(s) -
TengkuIdris Tengku Idzzan Nadzirah,
Fong Mun Yik,
Lau Yee Ling
Publication year - 2018
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/tmi.13160
Subject(s) - sarcocystosis , seroprevalence , western blot , biology , virology , toxoplasmosis , sarcocystis , immunology , serology , antibody , parasite hosting , biochemistry , world wide web , computer science , gene
Objective To investigate the seroprevalence of Sarcocystosis in the local communities of Pangkor and Tioman islands, Malaysia, by using antigenic recombinant surface antigens 2 and 3 from Sarcocystis falcatula ( rSfSAG 2 and rSfSAG 3) as the target proteins via Western blot and ELISA assays. Methods Sf SAG 2 and Sf SAG 3 genes were isolated from S. falcatula and expressed in Escherichia coli expression system. A total of 348 serum samples [volunteers from both islands ( n = 100), non‐ Sarcocystis parasitic infections patients ( n = 50) and healthy donors ( n = 100)] were collected and tested with purified Sf SAG s in Western blot and ELISA assays to measure the seroprevalence of human sarcocystosis. Results None of the sera in this study reacted with rSfSAG 2 by Western blot and ELISA . For rSfSAG 3, relatively high prevalence of sarcocystosis was observed in Tioman Island (75.5%) than in Pangkor Island (34%) by Western blot. In ELISA , the different prevalence rate was observed between Tioman Island (43.8%) and Pangkor Island (37%). The prevalence rate in other parasitic infections (amoebiasis, cysticercosis, filariasis, malaria, toxocariasis and toxoplasmosis) was 30% by Western blot and 26% by ELISA . Only 8% (by Western blot) and 10% (by ELISA ) of healthy donors showed reactivity towards rSfSAG 3. Conclusion This is the first study reporting a seroprevalence of sarcocystosis in Pangkor and Tioman Islands, Malaysia. The combination of Western blot and ELISA is suitable to be used for serodiagnosis of sarcocystosis. With further evaluations, Sf SAG 3 can potentially be used to confirm infection, asymptomatic screening, surveillance and epidemiological studies.