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Genetic diversity of Plasmodium falciparum merozoite surface protein‐1 (block 2), glutamate‐rich protein and sexual stage antigen Pfs25 from Chandigarh, North India
Author(s) -
Kaur Hargobinder,
Sehgal Rakesh,
Goyal Kapil,
Makkar Nikita,
Yadav Richa,
Bharti Praveen K.,
Singh Neeru,
Sarmah Nilanju P.,
Mohapatra Pradyumna K.,
Mahanta Jagadish,
Bansal Devendra,
Sultan Ali A.,
Kanwar Jagat R.
Publication year - 2017
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/tmi.12990
Subject(s) - malaria , plasmodium falciparum , genetic diversity , merozoite surface protein , biology , allele , sanger sequencing , genotype , genetic variation , nested polymerase chain reaction , genetics , virology , polymerase chain reaction , population , gene , dna sequencing , immunology , medicine , malaria vaccine , environmental health
Objective To elucidate the genetic diversity of Plasmodium falciparum in residual transmission foci of northern India. Methods Clinically suspected patients with malaria were screened for malaria infection by microscopy. 48 P. falciparum ‐infected patients were enrolled from tertiary care hospital in Chandigarh, India. Blood samples were collected from enrolled patients, genomic DNA extraction and nested PCR was performed for further species confirmation. Sanger sequencing was carried out using block 2 region of msp1, R2 region of glurp and pfs25 ‐specific primers. Results Extensive diversity was found in msp1 alleles with predominantly RO 33 alleles. Overall allelic prevalence was 55.8% for RO 33, 39.5% for MAD 20 and 4.7% for K1. Six variants were observed in MAD 20, whereas no variant was found in RO 33 and K1 alleles. A phylogenetic analysis of RO 33 alleles indicated more similarity to South African isolates, whereas MAD 20 alleles showed similarity with South‐East Asian isolates. In glurp, extensive variation was observed with eleven different alleles based on the AAU repeats. However, pfs25 showed less diversity and was the most stable among the targeted genes. Conclusion Our findings document the genetic diversity among circulating strains of P. falciparum in an area of India with low malaria transmission and could have implications for control strategies to reach the national goal of malaria elimination.