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Characterisation of clinical Staphylococcus aureus isolates harbouring mecA or Panton–Valentine leukocidin genes from four tertiary care hospitals in Indonesia
Author(s) -
Santosaningsih Dewi,
Santoso Sanarto,
Budayanti Nyoman S.,
Suata Ketut,
Lestari Endang S.,
Wahjono Hendro,
Djamal Aziz,
Kuntaman Kuntaman,
Belkum Alex,
Laurens Mitchell,
Snijders Susan V.,
WillemseErix Diana,
Goessens Wil H.,
Verbrugh Henri A.,
Severin Juliëtte A.
Publication year - 2016
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/tmi.12692
Subject(s) - staphylococcus aureus , panton–valentine leukocidin , leukocidin , tertiary care , microbiology and biotechnology , medicine , biology , methicillin resistant staphylococcus aureus , family medicine , bacteria , genetics
Objectives To determine the prevalence, antimicrobial susceptibility profiles and clonal distribution of either methicillin‐resistant Staphylococcus aureus ( MRSA ) or Panton–Valentine leukocidin ( PVL )‐positive S. aureus obtained from clinical cultures in Indonesian hospitals. Methods S. aureus isolates from clinical cultures of patients in four tertiary care hospitals in Denpasar, Malang, Padang and Semarang were included. We assessed the antimicrobial susceptibility profiles using the Vitek2 ® system, determined the presence of the mecA gene and genes encoding PVL using PCR and analysed the clonal relatedness with Raman spectroscopy. SCC mec typing was performed for all MRSA isolates. Multilocus sequence typing ( MLST ) was performed for a subset of isolates. Results In total, 259 S. aureus strains were collected. Of these, 17/259 (6.6%) and 48/259 (18.5%) were MRSA and PVL ‐positive methicillin‐susceptible S. aureus ( MSSA ), respectively. The prevalence of MRSA and PVL ‐positive MSSA ranged between 2.5–8.9% and 9.5–29.1%, respectively and depended on geographic origin. PVL ‐positive MRSA were not detected. Raman spectroscopy of the strains revealed multiple Raman types with two predominant clusters. We also showed possible transmission of a ST 239‐ MRSA ‐ SCC mec type III strain and a ST 121 PVL ‐positive MSSA in one of the hospitals. Conclusions We showed that MRSA and PVL ‐positive MSSA are of clinical importance in Indonesian hospitals. A national surveillance system should be set‐up to further monitor this. To reduce the prevalence of MRSA in Indonesian hospitals, a bundle of intervention measures is highly recommended.