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Immunoagglutination test to diagnose Chagas disease: comparison of different latex–antigen complexes
Author(s) -
Garcia Valeria S.,
Gonzalez Verónica D. G.,
Marcipar Ivan S.,
Gugliotta Luis M.
Publication year - 2014
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/tmi.12379
Subject(s) - chagas disease , antigen , medicine , immunology , virology
Objective To evaluate the diagnostic performance of novel latex–protein complexes obtained from different antigens of Trypanosoma cruzi through immunoagglutination test using a panel of T. cruzi ‐positive sera, leishmaniasis‐positive sera and negative sera for both parasites. Methods Complexes’ behaviour using total parasite homogenate ( TPH ), two simple recombinant proteins ( RP 1 and RP 5) and two chimeric recombinant proteins ( CP 1 and CP 2) was comparatively evaluated. The area under ROC curves was used as an index of accuracy. Sensitivity, specificity and discrimination efficiency were assessed. Results All recombinant antigens showed higher specificity than TPH . The lower specificity of TPH was mainly due to cross‐reacting peptides between T. cruzi and Leishmania spp. In turn, all performance indicators were higher for CP 1 and CP 2 than for RP 1 and RP 5. The carboxylated latex– CP 2 (C2– CP 2) complex was able to detect antibodies against T. cruzi . The values of area under ROC curve (0.96), sensitivity (92.3%, 95% CI : 79.4–100.0%) and specificity (84.0%, 95% CI : 67.6–100.0%) indicate that the assay could be used as a screening test. Conclusion The C2– CP 2 complex could be an important tool to carry out sero‐epidemiological studies.