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Rapid molecular screening for multidrug‐resistant tuberculosis in a resource‐limited region of China
Author(s) -
Zhang Dan,
Liu Beizhong,
Wang Yufeng,
Pang Yu
Publication year - 2014
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/tmi.12359
Subject(s) - tuberculosis , china , medicine , multiple drug resistance , virology , geography , biology , drug resistance , microbiology and biotechnology , pathology , archaeology
Objective To investigate the molecular characteristics of MDR and XDR strains circulating in Chongqing, China. Methods The drug target genes conferring for rifampicin ( RIF ), isoniazid ( INH ), ethambutol ( EMB ), ofloxacin ( OFLX ) and kanamycin ( KAN ) resistance were screened by DNA sequencing to determine the mutation frequencies in this area. Results Drug susceptibility of 208 MDR isolates revealed that 132 (63.46%) were resistant to streptomycin ( SM ), 96 (46.15%) to ethambutol ( EMB ), 51 (24.52%) to ofloxacin ( OFLX ), and 26 (12.50%) to kanamycin ( KAN ); six (2.88%) isolates had XDR profiles. In comparison with the drug susceptibility phenotype, the sensitivity of drug resistance by DNA sequencing was 91.83% for RIF , 87.50% for INH , 66.67% for EMB , 74.51% for OFLX and 53.85% for KAN resistance. 12.50% of EMB ‐ and 1.27% of OFLX ‐susceptible isolates were harboured genetic mutations in embB and gyrA , respectively. Conclusion Our findings demonstrate that the hot‐spot regions localised in rpoB , katG and inhA genes serve as excellent markers for the corresponding drug resistance, while EMB , OFLX or KAN drug‐resistant TB cases may not be identifiable by scanning embB , gyrA, rrs and eis promoter in Chongqing, indicating that further studies on the drug resistance mechanisms of EMB , OFLX and KAN are urgently needed to elucidate the low sensitivity between genomic substitutions and drug‐resistant phenotype.