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Ammonium improves elution of fixed dried blood spots without affecting immunofluorescence assay quality
Author(s) -
Borremans Benny
Publication year - 2014
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/tmi.12259
Subject(s) - ammonium , chromatography , elution , chemistry , antibody , dilution , immunofluorescence , dried blood , spots , ammonium sulfate , immunology , medicine , physics , organic chemistry , thermodynamics
Objective To solve the problem of fixed dried blood spot elution without damaging IgG antibodies. Methods The minimum effective concentration of liquid ammonium ( NH 3 ) in a PBS solution, which was found to elute fixed blood, was determined. By using a dilution series, the effects of NH 3 on IgG antibody quality were assessed using immunofluorescence assays. Results The minimum effective concentration of 0.2% NH 3 has no detectable effects on IgG quality. Conclusion Ammonium greatly improves blood elution from fixed DBS while maintaining IgG antibody quality. These results are encouraging and provide a basis for further testing of the efficacy of ammonium in different situations as well as its effect on other compounds.