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First report of detection and molecular confirmation of P lasmodium ovale from severe malaria cases in central I ndia
Author(s) -
Singh R.,
Jain V.,
Singh P. P.,
Bharti P. K.,
Thomas T.,
Basak S.,
Singh N.
Publication year - 2013
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/tmi.12184
Subject(s) - plasmodium ovale , malaria , plasmodium falciparum , cerebral malaria , plasmodium vivax , medicine , genotype , virology , biology , immunology , genetics , gene
Abstract Objective We report for the first‐time detection of P lasmodium ovale in central I ndia. Methods From 2010, all hospitalised suspected malaria cases at the malaria clinic of the R egional M edical R esearch C entre for T ribals in B astar district, central I ndia, were screened. Plasmodium species were identified by microscopy and species‐specific nested PCR of 18s rRNA . Results Of 256 enrolled cases of confirmed P . falciparum malaria by microscopy, P . ovale infection was detected in three cases (1.2%) by PCR and sequencing. Of these three cases, one had cerebral malaria and another had severe malaria anaemia. In both of these cases, P . ovale infection was mixed with P. falciparum, while in third case the infection was mixed with both P . falciparum and P . vivax . Phylogenetic analysis revealed that these isolates showed closed homology with W est A frican genotypes. Conclusion All three hospitalised patients were originally residents of remote inaccessible forest villages and never moved out of their residence. This finding also has implications in malaria control and elimination as P . ovale causes relapses. This study highlights the need of molecular diagnosis of malaria species for appropriate treatment and control.