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The screening of rare blood donors in a highly admixed population: A new approach for Holley and Diego genotyping and impact of genomic and self‐reported ancestry
Author(s) -
Muniz Amanda A.,
Silva Adão R.,
Ferraz Isabela A.,
Martins Marina L.,
Godin Mariana M.,
Schmidt Luciana C.,
Dusse Luci M. S. A.,
Silva Malta Maria Clara Fernandes
Publication year - 2020
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/tme.12653
Subject(s) - genotyping , genotype , allele , population , polymerase chain reaction , biology , genomic dna , phenotype , ancestry informative marker , genetics , allele frequency , medicine , gene , environmental health
Summary Objectives The present study aimed to develop strategies for genotyping DO*HY (Dombrock system) and DI*A/DI*B (Diego system) alleles and to evaluate the impact of genomic and self‐declared ancestry on rare donor screening in admixed populations. Background The antigens Hy and Di b demonstrate clinical importance. The lack of antisera for the serological evaluation of these antigens makes it necessary to develop molecular methods. In addition, considering that some rare red blood cell phenotypes present differences in frequency between ethnic groups, it is important to assess the applicability of self‐declared ancestry in the search for rare donors in admixed populations. Methods DO*HY and DI*A/DI*B genotyping based on real‐time polymerase chain reaction (PCR) was standardised. A total of 457 blood donors clustered by self‐defined skin colour/race categories were genotyped. Furthermore, individual genomic ancestry was used in the analyses. Results The assays developed are reproducible and provide satisfactory results even at low concentrations of DNA, which make them useful in situations where the DNA is scarce, such as dried blood spots on filter paper, or when screening for pooled samples. No significant difference was observed in the frequencies of the DI*A , DI*B and DO*HY , comparing the self‐declared White (branco) donors with those who are Black (preto) and Brown (pardo). Conclusion Real‐time PCR, especially using pooled samples, is a promising strategy to screen rare blood donors. Although both self‐reported race/colour and some blood group phenotypes are associated with ancestry, the results point to a greater complexity in the application of self‐declared race/colour in the screening of rare donors in admixed populations.