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In vitro quality of apheresis platelets divided into paediatric‐sized units and stored in PVC bags plasticised with TOTM, BTHC or DINCH
Author(s) -
Bashir S.,
Meli A.,
Cardigan R.
Publication year - 2018
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/tme.12528
Subject(s) - apheresis , plastic bag , platelet , plateletpheresis , medicine , food science , chemistry , waste management , immunology , engineering
SUMMARY Background and Objectives Two of the predictive factors of the quality of small volumes of platelets suitable for paediatric use are bag size and material. This study evaluated the storage properties of paediatric platelet aliquots in TOTM‐, BTHC‐ or DINCH‐PVC bags. Methods/Materials (i) Three apheresis platelet concentrates (PC) were pooled and split into three units. One was retained as an adult unit (control; polyolefin bag). The second and third units were split into four MacoPharma TOTM‐PVC and BTHC‐PVC paediatric bags, respectively. (ii) Two apheresis PC were pooled and split into two units. One PC was retained as an adult unit, and the other was split into four Fresenius DINCH‐PVC paediatric bags. Testing was performed on storage for pH, blood gases, hypotonic shock response, soluble CD62P, LDH, glucose and lactate, ATP, CD62P, CD63, platelet‐derived microparticles and annexin V. Results The volumes, platelet yields and pH of all paediatric units met local specifications. The TOTM‐PVC bag showed no worse quality than the adult bag up to day 7 for all parameters studied, and it maintained pH higher than BTHC‐PVC and DINCH‐PVC over storage. The BTHC‐PVC bag was shown to be the most gas permeable; however, it had the highest glucose consumption rates and the highest platelet activation. Conclusion All bags showed an acceptable in vitro quality. Overall, the TOTM‐PVC paediatric bag showed better platelet quality compared to the other storage bags, whereas storage in the BTHC‐PVC bag resulted in poorer platelet quality.