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Quantitative norovirus viral load is not affected by home storage of stool
Author(s) -
Ison Michael G.,
Tan Ming,
Daud Amna,
Huang Pengwei,
Jiang Jason Xi
Publication year - 2022
Publication title -
transplant infectious disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.69
H-Index - 67
eISSN - 1399-3062
pISSN - 1398-2273
DOI - 10.1111/tid.13826
Subject(s) - norovirus , medicine , murine norovirus , viral load , virology , virus quantification , microbiology and biotechnology , virus , biology
In preparation of a clinical trial of norovirus treatment, there were concerns raised by FDA about risk of self‐storage of stool from patients infected with norovirus affecting quantitative assessments of norovirus RNA. Specifically, most home freezers are frost‐free and may expose the samples to multiple rounds of freeze‐thaw. Stool samples collected by the study team were stored at different lengths in a frost‐free freezer and at −80°C. Quantitative PCRs of norovirus were performed on all samples using the same assay. By all measures, there was no significant change in measured viral load with home storage.