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Human papillomavirus detected in viral warts of renal transplant recipients
Author(s) -
MartelliMarzagão F.,
Santos Junior G.F.,
Ogawa M.M.,
Enokihara M.M.S.S.,
Porro A.M.,
Tomimori J.
Publication year - 2016
Publication title -
transplant infectious disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.69
H-Index - 67
eISSN - 1399-3062
pISSN - 1398-2273
DOI - 10.1111/tid.12479
Subject(s) - genotyping , typing , medicine , epidermodysplasia verruciformis , virology , renal transplant , population , human papillomavirus , hpv infection , polymerase chain reaction , papillomaviridae , genotype , virus , transplantation , biology , microbiology and biotechnology , cervical cancer , genetics , cancer , gene , environmental health
Objectives Few studies have been conducted in South America regarding the detection and genotyping of human papillomavirus ( HPV ) in viral warts of renal transplant recipients ( RTRs ). The characterization of the population most susceptible to the development of warts and the knowledge of the main HPV types in this environment prompted this study, which focuses on the detection and typing of HPV in RTR s in Brazil. Methods Fifty‐eight patients with viral warts from the Hospital São Paulo/Federal University of São Paulo were included in this study. HPV was detected by polymerase chain reaction ( PCR ) using combinations of the following primers: PGMY 09/11, RK 91, CP 65/70, and CP 66/69. Restriction fragment length polymorphism and automated sequencing techniques were used for HPV typing. Results HPV was detected by PCR in 89.7% of viral wart samples. The most frequently detected HPV types included 57, 27, 1a, 2a, and 20. Other types of HPV ‐epidermodysplasia verruciformis were also detected, including 14, 15, 19, 20, 21, 23, 36, and 38. Rare HPV types were also detected in our environment, including RTR X1, RTR X7, and 100. The time after transplant was correlated with an increased number of lesions and beta papillomavirus genus infection. Conclusions The HPV types detected in the RTR population were similar to those described in immunocompetent populations. However, the diversity of the HPV types identified and the number of lesions were increased in the RTR population.