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Persistence of recipient‐derived as well as donor‐derived clones of cytomegalovirus pp65‐specific cytotoxic T cells long after allogeneic hematopoietic stem cell transplantation
Author(s) -
TerasakoSaito K.,
Nakasone H.,
Tanaka Y.,
Yamazaki R.,
Sato M.,
Sakamoto K.,
Ishihara Y.,
Kawamura K.,
Akahoshi Y.,
Hayakawa J.,
Wada H.,
Harada N.,
Nakano H.,
Kameda K.,
Ugai T.,
Yamasaki R.,
Ashizawa M.,
Kimura S.I.,
Kikuchi M.,
Tanihara A.,
Kanda J.,
Kako S.,
Nishida J.,
Kanda Y.
Publication year - 2014
Publication title -
transplant infectious disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.69
H-Index - 67
eISSN - 1399-3062
pISSN - 1398-2273
DOI - 10.1111/tid.12318
Subject(s) - ctl* , cytotoxic t cell , clone (java method) , immunology , virology , transplantation , cytomegalovirus , hematopoietic stem cell transplantation , biology , stem cell , medicine , virus , immune system , cd8 , herpesviridae , gene , viral disease , genetics , in vitro , surgery
Background Cytomegalovirus ( CMV )‐specific CD 8 + cytotoxic T lymphocytes ( CMV ‐ CTL s) play a crucial role in preventing CMV disease. However, the actual in vivo dynamics of CMV ‐ CTL clones after allogeneic hematopoietic stem cell transplantation (allo HCT ) are still unclear. Methods Using a single‐cell T‐cell receptor repertoire analysis, we monitored clones and chimerism of CMV ‐ CTL s in 3 CMV ‐seropositive allo HCT recipients from CMV ‐seronegative donors, with or without CMV reactivation. Results Nearly all of the CMV ‐ CTL s during follow‐up were CD 45 RA − CCR 7 − effector memory/ CD 45 RA + CCR 7 − effector T cells, and were highly matured. In each case, the use of BV gene families was restricted, especially in BV 5, 7, 28, and 29. Although no common predominant CMV ‐ CTL clones were found, several shared motifs of complementarity‐determining region‐3 were identified among the 3 cases; QGA in all, TGE and TDT in Case 1 and Case 2, and RDRG in Case 2 and Case 3. In all cases, CMV ‐ CTL clones that were detected for the first time after allo HCT persisted as the dominant clones. In Case 1, without CMV reactivation, recipient‐derived CMV ‐ CTL s exclusively persisted as a dominant clone, while all CMV ‐ CTL s in the other 2 cases, with CMV reactivation, were donor derived. Conclusion Clone monitoring and chimerism analyses should help to further clarify novel aspects of immuno‐reconstitution after allo HCT .