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Cord blood graft composition impacts the clinical outcome of allogeneic stem cell transplantation
Author(s) -
Wikell H.,
PonandaiSrinivasan S.,
Mattsson J.,
Gertow J.,
Uhlin M.
Publication year - 2014
Publication title -
transplant infectious disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.69
H-Index - 67
eISSN - 1399-3062
pISSN - 1398-2273
DOI - 10.1111/tid.12182
Subject(s) - medicine , stem cell , transplantation , cd8 , hematopoietic stem cell transplantation , immunology , umbilical cord , cord blood , graft versus host disease , immune system , biology , genetics
Despite routine use of umbilical cord blood ( CB ) grafts as stem cell source for allogeneic stem cell transplantations, much remains unknown regarding their cell composition and correlation with clinical outcome. Methods We analyzed material from 30 CB units used for allogeneic hematopoietic stem cell transplantation by multicolor flow cytometry. Phenotypic data were correlated with various clinical outcomes such as survival, graft‐versus‐host disease ( GVHD ), relapse, rejection, viral reactivation, and bacteremia. Results We found that above‐median frequencies of CD 69+ T cells, naïve CD 8+ T cells, and CD 127+ B cells in the CB graft were each associated with significantly improved patient survival. Moreover, a statistically significant correlation was seen between higher levels of CD 94+ T cells and herpes simplex virus and varicella zoster virus reactivation post transplantation. A similar correlation was seen for the frequency of CD 95+ cells in total CD 3+, as well as CD 4+ and CD 8+ T‐cell subsets, and viral reactivation. Finally, a higher frequency of naïve CD 8+ T cells was associated with the incidence of acute GVHD . Conclusion Our study highlights the importance of further exploration of graft composition before CB transplantation as a tool for risk prediction.