Premium
Inactivation of H‐type and L‐type bovine spongiform encephalopathy following recommended autoclave decontamination procedures
Author(s) -
Chapman Gail E.,
Lockey Richard,
Beck Katy E.,
Vickery Chris,
Arnold Mark,
Thorne Leigh,
Thorne Jemma K.,
Walker Sarah R.,
Keulen Lucien,
Casalone Cristina,
Griffiths Peter C.,
Simmons Marion M.,
Terry Linda A.,
Spiropoulos John
Publication year - 2020
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.13513
Subject(s) - bovine spongiform encephalopathy , infectivity , human decontamination , titer , incubation period , virology , bioassay , incubation , microbiology and biotechnology , biology , chemistry , medicine , prion protein , virus , biochemistry , genetics , disease , pathology
The resistance of H‐type and L‐type BSE prions to autoclaving under EU regulation conditions for specified risk material is unknown. We employed transgenic mouse (bovinized line tg1896) bioassay to assess the efficacy of such decontamination on L‐ and H‐type BSE. For each source, titre calculation was based on the comparison of incubation period and attack rate prior to and after decontamination. The infectious titre of L‐type BSE was reduced by at least 9.40 log 10 and of H‐type BSE by at least 3.94 log 10 . In fact, no infectivity was detected for L‐type or H‐type BSE post‐inactivation even at a 10 –1 dilution.