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A bivalent B‐cell epitope dendrimer peptide can confer long‐lasting immunity in swine against foot‐and‐mouth disease
Author(s) -
CañasArranz Rodrigo,
Forner Mar,
Defaus Sira,
RodríguezPulido Miguel,
León Patricia,
Torres Elisa,
Bustos María J.,
Borrego Belén,
Sáiz Margarita,
Blanco Esther,
Andreu David,
Sobrino Francisco
Publication year - 2020
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.13497
Subject(s) - epitope , virology , foot and mouth disease virus , immune system , biology , bivalent (engine) , t cell , antibody , virus , immunity , immunology , chemistry , organic chemistry , metal
Foot‐and‐mouth disease virus (FMDV) causes a widely extended contagious disease of livestock. We have previously reported that a synthetic dendrimeric peptide, termed B 2 T(mal), consisting of two copies of a B‐cell epitope [VP1(140–158)] linked through maleimide groups to a T‐cell epitope [3A(21–35)] of FMDV, elicits potent B‐ and T‐cell‐specific responses and confers solid protection in pigs to type O FMDV challenge. Longer duration of the protective response and the possibility of inducing protection after a single dose are important requirements for an efficient FMD vaccine. Herein, we show that administration of two doses of B 2 T(mal) elicited high levels of specific total IgGs and neutralizing antibodies that lasted 4–5 months after the peptide boost. Additionally, concomitant levels of IFN‐γ‐producing specific T cells were observed. Immunization with two doses of B 2 T(mal) conferred a long‐lasting reduced susceptibility to FMDV infection, up to 136 days (19/20 weeks) post‐boost. Remarkably, a similar duration of the protective response was achieved by a single dose of B 2 T(mal). The effect on the B 2 T(mal) vaccine of RNA transcripts derived from non‐coding regions in the FMDV genome, known to enhance the immune response and protection induced by a conventional inactivated vaccine, was also analysed. The contribution of our results to the development of FMD dendrimeric vaccines is discussed.