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Serological and phylogenetic characterization of foot and mouth disease viruses from Uganda during cross‐sectional surveillance study in cattle between 2014 and 2017
Author(s) -
Mwiine Frank Norbert,
VelazquezSalinas Lauro,
Ahmed Zaheer,
Ochwo Sylvester,
Munsey Anna,
Kenney Mary,
Lutwama Julius J.,
Maree Francois F.,
Lobel Leslie,
Perez Andres M.,
Rodriguez Luis L.,
VanderWaal Kimberly,
Rieder Elizabeth
Publication year - 2019
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.13249
Subject(s) - serotype , foot and mouth disease , virology , phylogenetic tree , biology , foot and mouth disease virus , serology , genetic diversity , seroprevalence , livestock , veterinary medicine , molecular epidemiology , virus , population , antibody , genotype , genetics , medicine , ecology , gene , environmental health
Here, we report the results of a cross‐sectional study designed to monitor the circulation and genetic diversity of foot and mouth disease virus (FMDV) in Uganda between 2014 and 2017. In this study, 13,614 sera and 2,068 oral‐pharyngeal fluid samples were collected from cattle and analysed to determine FMDV seroprevalence, circulating serotypes and their phylogenetic relationships. Circulation of FMDV was evidenced by the detection of antibodies against non‐structural proteins of FMDV or viral isolations in all districts sampled in Uganda. Sequence analysis revealed the presence of FMDV serotypes A, O, SAT 1 and SAT 2. FMDVs belonging to serotype O, isolated from 21 districts, were the most prevalent and were classified into six lineages within two East African topotypes, namely EA‐1 and EA‐2. Serotype A viruses belonging to the Africa G‐I topotype were isolated from two districts. SAT 1 viruses grouped within topotypes I and IV and SAT 2 viruses within topotypes VII, IV and X were isolated from six and four districts respectively. Phylogenetic analysis of SAT 1 and SAT 2 sequences from cattle clustered with historical sequences from African buffalo, indicating possible interspecies transmission at the wildlife‐livestock interface. In some cases, Uganda viruses also shared similarities to viral strains recovered from other regions in East Africa. This 3‐year study period provides knowledge about the geographical distribution of FMDV serotypes isolated in Uganda and insights into the genetic diversity of the multiple serotypes circulating in the country. Knowledge of circulating FMDV viruses will assist in antigenic matching studies to devise improved FMDV control strategies with vaccination and vaccine strain selection for Uganda.