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Western Bluetongue virus serotype 3 in Sardinia, diagnosis and characterization
Author(s) -
Cappai S.,
Rolesu S.,
Loi F.,
Liciardi M.,
Leone A.,
Marcacci M.,
Teodori L.,
Mangone I.,
Sghaier S.,
Portanti O.,
Savini G.,
Lorusso A.
Publication year - 2019
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.13156
Subject(s) - virology , serotype , orbivirus , virus , biology , geography , reoviridae , rotavirus
Over the last 20 years, Italy has experienced multiple incursions of different serotypes of Bluetongue virus ( BTV ), a Culicoides ‐borne arbovirus, the causative agent of bluetongue ( BT ), a major disease of ruminants. The majority of these incursions originated from Northern Africa, likely because of wind‐blown dissemination of infected midges. Here, we report the first identification of BTV ‐3 in Sardinia, Italy. BTV ‐3 circulation was evidenced in sentinel animals located in the province of Sud Sardegna on September 19, 2018. Prototype strain BTV ‐3 SAR 2018 was isolated on cell culture. BTV ‐3 SAR 2018 sequence and partial sequences obtained by next‐generation sequencing from nucleic acids purified from the isolate and blood samples, respectively, were demonstrated to be almost identical (99–100% of nucleotide identity) to BTV ‐3 TUN 2016 identified in Tunisia in 2016 and 2017, a scenario already observed in past incursions of other BTV serotypes originating from Northern Africa.