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Elizabethkingia miricola infection in Chinese spiny frog ( Quasipaa spinosa )
Author(s) -
Lei Xue Ping,
Yi Geng,
Wang Kai Yu,
OuYang Ping,
Chen De Fang,
Huang Xiao Li,
Huang Chao,
Lai Wei Min,
Zhong Zi Jun,
Huo Chang Liang,
Yang Ze Xiao
Publication year - 2019
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.13101
Subject(s) - biology , pathogen , 16s ribosomal rna , microbiology and biotechnology , spleen , bacteria , botany , genetics , immunology
Abstract Elizabethkingia miricola is a Gram‐negative rod which has been incriminated in severe infections in humans. Recently, a serious infectious disease was identified in Chinese spiny frogs ( Quasipaa spinosa ), in the Sichuan Province of China; the disease was characterized by corneal opacity, the presence of ascites and neurological symptoms. A Gram‐negative bacillus was isolated from the liver, spleen and kidney of the diseased frogs. Experimental infection test revealed that the bacillus could infect the frogs Q. spinosa and the LD 50 value was 1.19 × 10 6 cfu per frog. The isolated Gram‐negative bacillus was identified as E . miricola according to phenotypic characteristics, 16S rRNA and gyr B gene sequence analysis. The isolated strain was only susceptible to florfenicol among all investigated chemotherapeutic agents. Histological examination revealed that E. miricola infection caused pathological lesions to multiple organs and tissues, especially in the liver, brain, kidney. These results confirmed that E. miricola is an emerging pathogen of Chinese spiny frogs.