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Phylogenetic variations of highly pathogenic H5N6 avian influenza viruses isolated from wild birds in the Izumi plain, Japan, during the 2016–17 winter season
Author(s) -
Ozawa Makoto,
Matsuu Aya,
Khalil Ahmed Magdy,
Nishi Natsuko,
Tokorozaki Kaori,
Masatani Tatsunori,
Horie Masayuki,
Okuya Kosuke,
Ueno Kosei,
Kuwahara Masakazu,
Toda Shigehisa
Publication year - 2019
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.13087
Subject(s) - influenza a virus subtype h5n1 , phylogenetic tree , biology , overwintering , virology , phylogenetics , winter season , highly pathogenic , genotype , gene , zoology , feces , virus , genetics , ecology , climatology , geology
During the 2016–2017 winter season, we isolated 33 highly pathogenic avian influenza viruses ( HPAIV s) of H5N6 subtype and three low pathogenic avian influenza viruses ( LPAIV s) from debilitated or dead wild birds, duck faeces, and environmental water samples collected in the Izumi plain, an overwintering site for migratory birds in Japan. Genetic analyses of the H5N6 HPAIV isolates revealed previously unreported phylogenetic variations in the PB 2, PB 1, PA , and NS gene segments and allowed us to propose two novel genotypes for the contemporary H5N6 HPAIV s. In addition, analysis of the four gene segments identified close phylogenetic relationships between our three LPAIV isolates and the contemporary H5N6 HPAIV isolates. Our results implied the co‐circulation and co‐evolution of HPAIV s and LPAIV s within the same wild bird populations, thereby highlighting the importance of avian influenza surveillance targeting not only for HPAIV s but also for LPAIV s.