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Generation of monoclonal antibodies against foot‐and‐mouth disease virus SAT 2 and the development of a lateral flow strip test for virus detection
Author(s) -
Yang Ming,
Mudabuka Boitumelo,
Quizon Kaye,
Nfon Charles
Publication year - 2019
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.13076
Subject(s) - monoclonal antibody , virology , outbreak , foot and mouth disease virus , serotype , foot and mouth disease , antibody , virus , biology , medicine , immunology
Foot‐and‐mouth disease ( FMD ) remains a major economic concern for the livestock productivity in many developing countries and a continued threat to countries that are disease free because of its potential devastating impact on agricultural, food chain and tourism sectors. FMD virus ( FMDV ) is recognized as having seven serotypes: O, A, C, Asia 1, South African Territories ( SAT ) 1, 2, 3 and multiple subtypes within each serotype. FMD outbreaks due to SAT 2 have been reported in many African countries. The development of a rapid and easily performed test for FMD detection is critical for controlling FMD outbreaks and containing its spread. The present project developed a lateral flow immunochromatographic ( LFI ) strip test for the rapid detection of FMDV SAT 2. A panel of monoclonal antibodies ( mA bs) against FMDV serotype SAT 2 was produced and characterized. One mAb (#10) was selected as the capture mAb because it reacted to all 23 SAT 2 isolates archived at the National Center for Foreign Animal Disease. The LFI strip test was developed using biotin‐conjugated mA b #10, and the colloid gold‐conjugated FMDV serotype‐independent mAb as the detection mAb. A generic Rapid Assay Device (gRAD) with one test line and a control line was used for the test. The LFI strip test detected all 23 tested SAT 2 isolates and recent outbreak strains. The results indicated that the diagnostic specificity and sensitivity of the LFI strip test were greater than the double antibody sandwich ( DAS ) DAS ELISA . The ability of the LFI strip test to produce rapid diagnostic results will be useful for early on‐site diagnosis during FMD outbreaks.