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First report and genetic characterization of porcine astroviruses of lineage 4 and 2 in diarrhoeic pigs in India
Author(s) -
Kattoor Jobin Jose,
Malik Yashpal Singh,
Saurabh Sharad,
Sircar Shubhankar,
Vinodhkumar Obli Rajendran,
Bora Durlav Prasad,
Dhama Kuldeep,
Ghosh Souvik,
Banyai Krisztian,
Touil Nadia,
AbdelMoneim Ahmed S.,
Vlasova Anastasia N.,
Kobayashi Nobumichi,
Singh Raj Kumar
Publication year - 2019
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.13058
Subject(s) - lineage (genetic) , phylogenetic tree , biology , genetic diversity , population , phylogenetics , genetics , sequence analysis , rotavirus , virology , genetic analysis , gene , virus , demography , sociology
Summary Porcine astroviruses ( PA stVs) have extended their distribution globally and have a high prevalence; however, their clinical significance is still under investigation. Thus far, information about their prevalence and diversity in the Indian pig population is unknown. This study is the first report on the prevalence and genetic characterization of PA stVs in diarrhoeic piglets in India. From January 2013 to December 2017, 757 samples were screened using an RT ‐ PCR assay and PA stV infection was detected in 17.6% (133/757) pigs. Of the 133 positive samples, 79 (59.4%) were positive for PA stV alone, whereas 54 (40.6%) were found to be co‐infected with porcine rotavirus A (Po RVA ). Phylogenetic analysis of RdRp/capsid gene region revealed high genetic heterogeneity among PA stV sequences, with a predominance of PA stV lineage 4 and detection of lineage 2. The lineage 4 PA stVs exhibited 61.2%–94.5% sequence similarity at the nucleotide level to other reported sequences, whereas lineage 2 strain shared 66.0%–71.6% sequence identity with cognate sequences of the same lineage. This is the first report on PA stV and circulation of lineages 4 and 2 in India. Further, phylogenetic analysis indicates a multiphyletic origin of PA stV strains and suggests cross‐border circulation of PA stVs with a similar genetic configuration in Asian countries.