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Isolation and genomic characterization of gosling gout caused by a novel goose astrovirus
Author(s) -
Yang Jing,
Tian Jiajun,
Tang Yi,
Diao Youxiang
Publication year - 2018
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12928
Subject(s) - astrovirus , goose , biology , virology , phylogenetic tree , genome , whole genome sequencing , genetics , virus , gene , ecology , rotavirus
Abstract A severe infectious disease characterized with gout, haemorrhage and swellings of kidneys has affected goslings around the major goose‐producing regions in China since November 2016. A Novel goose‐origin astrovirus ( AS tV), designated as AS tV/ SDPY /Goose/1116/17 ( AS tV‐ SDPY ) strain, was isolated from diseased goslings, and experimental reproduction of gout was successful using the AS tV‐ SDPY strain. Additionally, the AS tV‐ SDPY was conducted for its full genome sequencing characterization using next‐generation sequencing ( NGS ) technique on Illumina HiSeq platform. A complete genome of the AS tV‐ SDPY was 7,252 nt in length and encoded three viral proteins. Phylogenetic analysis revealed that AS tV‐ SDPY strain belongs to an independent branch of avian astroviruses, and the nucleotide homology among AS tV‐ SDPY and other classic avian astrovirus strains was only 48.8%–68.2%. Results of above data indicated the causative agent of the gosling gout occurring in China is a novel divergent goose astrovirus.