Premium
Full‐genome sequencing of porcine circovirus 3 field strains from Denmark, Italy and Spain demonstrates a high within‐Europe genetic heterogeneity
Author(s) -
Franzo G.,
Legnardi M.,
Hjulsager C. K.,
Klaumann F.,
Larsen L. E.,
Segales J.,
Drigo M.
Publication year - 2018
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12836
Subject(s) - epidemiology , porcine circovirus , genome , danish , biology , public health , epidemiological surveillance , transmission (telecommunications) , circovirus , veterinary medicine , virology , genetics , medicine , gene , pathology , linguistics , virus , philosophy , electrical engineering , engineering
Summary Porcine circovirus 3 ( PCV 3) is a new species of the Circovirus genus, which has recently been associated with different clinical syndromes. Its presence has been reported in different countries of North and South America, Asia and recently also Europe (Poland). However, different from the other continents, no European PCV 3 sequence is currently available in public databases. There is a strong need of epidemiological data and full‐genome sequences from Europe because of its relevance in the understanding of PCV 3 molecular epidemiology and control. To fill this lack of information, samples collected in Denmark, Italy and Spain in 2016 and 2017 were screened for PCV 3. Of the Danish samples, 36 of 38 the lymph nodes, six of 20 serum samples and two of 20 lung samples tested positive. Similarly, 10 of 29 lungs, 20 of 29 organ pools, six of 33 sera and one of eight nasal swabs tested PCV 3 positive in Italy. Fourteen of 94 serum pools from seven of 14 Spanish farms were also positive. Despite the convenience nature of the sampling prevents any precise prevalence estimation, the preliminary screening of the data from three European countries confirmed a rather wide PCV 3 distribution in Europe. Furthermore, the analysis of the six obtained complete European PCV 3 genomes and their comparison with the public available sequences seems to support a remarkable worldwide PCV 3 circulation. These results underline once more the urgency of more extensive epidemiological studies to refine the current knowledge on PCV 3 evolution, transmission, spreading patterns and impact on pig health.