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Isolation and phylogenetic analysis of porcine deltacoronavirus from pigs with diarrhoea in Hebei province, China
Author(s) -
Liu B.J.,
Zuo Y.Z.,
Gu W.Y.,
Luo S.X.,
Shi Q.K.,
Hou L.S.,
Zhong F.,
Fan J.H.
Publication year - 2018
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12821
Subject(s) - isolation (microbiology) , china , phylogenetic tree , biology , phylogenetic relationship , veterinary medicine , geography , virology , microbiology and biotechnology , genetics , medicine , gene , archaeology
Summary Porcine deltacoronavirus ( PDC oV) is a recently identified coronavirus in the genus Deltacoronavirus that can cause enteric disease with clinical signs including diarrhoea, vomiting, dehydration and mortality in neonatal piglets. Although evidence of the prevalence of PDC oV in China is accumulating, little published information about Chinese PDC oV isolates is available. In this study, we investigated the presence of PDC oV in 49 faecal/intestinal samples from piglets with diarrhoea on different farms in Hebei province. Five samples (10.2%) were positive for PDC oV, but no coinfection of PDC oV with other enteropathogens was observed. A PDC oV strain named HB ‐ BD was successfully isolated from the intestinal contents of a diarrhoeic piglet and serially propagated in swine testicular ( ST ) cells for >40 passages. The complete genome of the HB ‐ BD strain was sequenced and analysed. Genomic analysis showed that the HB ‐ BD strain had a closer relationship with Chinese strains than those from other countries and was grouped within the Chinese PDC oV cluster. The results of this study will be valuable for further research of PDC oV genetic evolution and development of effective diagnostic reagents, assays and potential vaccines against newly emerged PDC oV strains.