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Development of a chemiluminescence immunoassay using recombinant non‐structural epitope‐based proteins to accurately differentiate foot‐and‐mouth disease virus‐infected and vaccinated bovines
Author(s) -
Liu Z.Z.,
Zhao F.R.,
Gao S.D.,
Shao J.J.,
Zhang Y.G.,
Chang H.Y.
Publication year - 2018
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12811
Subject(s) - virology , foot and mouth disease virus , foot and mouth disease , immunoassay , virus , recombinant dna , epitope , biology , antibody , chemiluminescence , medicine , immunology , chemistry , chromatography , biochemistry , gene
Summary The contamination of inactivated vaccine with non‐structural proteins ( NSP s) leads to a high false‐positive rate, which is a substantial barrier to accurately differentiate foot‐and‐mouth disease virus ( FMDV )‐infected animals from vaccinated animals. To address this problem, a new chemiluminescence immunoassay ( CLIA ) method was developed to detect antibodies targeting the two recombinant epitope‐based proteins located in 3A and 3B. The 3Aepitp‐3Bepitp CLIA exhibited a diagnostic sensitivity of 94.0% and a diagnostic specificity of 97.5% for the detection of serum samples (naïve bovines, n = 52, vaccinated bovines, n = 422, infected bovines, n = 116) from animals with known status. The CLIA method also had a concordance rate of 88.1% with the Prio CHECK FMDV NSP ELISA based on the detection of 270 serum samples from the field. Importantly, the 3Aepitp‐3Bepitp CLIA produced no false‐positives when used to detect FMDV in samples from bovines that had been vaccinated up to five times, and it was demonstrated a low false‐positive rate when the bovines had been vaccinated up to ten (2.15%) and fifteen times (5.93%). Therefore, the 3Aepitp‐3Bepitp CLIA detects FMDV in samples from frequently vaccinated bovines with high accuracy and represents an alternative method to differentiate FMDV ‐infected and vaccinated bovines.