Premium
A G3P[13] porcine group A rotavirus emerging in China is a reassortant and a natural recombinant in the VP 4 gene
Author(s) -
Jing Z.,
Zhang X.,
Shi H.,
Chen J.,
Shi D.,
Dong H.,
Feng L.
Publication year - 2018
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12756
Subject(s) - reassortment , virology , recombinant dna , rotavirus , biology , gene , genetics , strain (injury) , virus , group a , genome , disease , medicine , covid-19 , pathology , anatomy , infectious disease (medical specialty)
Summary Group A rotaviruses ( RVA s) are a major cause of serious intestinal disease in piglets. In this study, a novel pig strain was identified in a stool sample from China. The strain was designated RVA /Pig/China/ LNCY /2016/G3P[13] and had a G3‐P[13]‐I5‐R1‐C1‐M1‐A8‐N1‐T1‐E1‐H1 genome. The viral protein 7 ( VP 7) and non‐structural protein 4 ( NSP 4) genes of RVA /Pig/China/ LNCY /2016/G3P[13] were closely related to cogent genes of human RVA s, suggesting that a reassortment between pig and human strains had occurred. Recombination analysis showed that RVA /Pig/China/ LNCY /2016/G3P[13] is a natural recombinant strain between the P[23] and P[7] RVA strains, and crossover points for recombination were found at nucleotides (nt) 456 and 804 of the VP 4 gene. Elucidating the biological characteristics of porcine rotavirus (Po RV ) will be helpful for further analyses of the epidemic characteristics of this virus. The results of this study provide valuable information for RVA recombination and evolution and will facilitate future investigations into the molecular pathogenesis of RVA s.