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Introduction of Asian canine parvovirus in Europe through dog importation
Author(s) -
Mira F.,
Purpari G.,
Lorusso E.,
Di Bella S.,
Gucciardi F.,
Desario C.,
Macaluso G.,
Decaro N.,
Guercio A.
Publication year - 2018
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12747
Subject(s) - biology , phylogenetic tree , canine parvovirus , virology , genome , virus , gene , strain (injury) , genetic analysis , orfs , sequence analysis , genetics , parvovirus , open reading frame , peptide sequence , anatomy
Summary Canine parvovirus ( CPV ) is an important infectious agent of domestic and wild carnivores, responsible for severe and often fatal haemorrhagic gastroenteritis and leukopenia. This paper reports the genomic characterization of a CPV strain collected from a dog recently imported to Italy from Thailand. The virus was detected in all tissue samples collected. The whole genome encompassing the two open reading frames encoding for non‐structural ( NS 1/ NS 2) and structural ( VP 1/ VP 2) proteins was amplified and sequenced. On the basis of genetic analysis of the VP 2 gene, the isolate was characterized as CPV ‐2c, but it presented genetic signatures typical of Asian strains. Sequence analysis revealed the presence of amino acid changes never observed in European CPV ‐2c strains ( NS 1: Ile60Val, Tyr544Phe, Glu545Val, Leu630Pro; VP 2: Ala5Gly, Phe267Tyr, Tyr324Ile, Gln370Arg). By phylogenetic analysis of full‐length VP 2 gene, the analysed strain clustered together with Asian viruses. Therefore, a possible introduction of the virus from Asia through the imported dog was suggested, thus confirming the important role of movement of dogs in the global spread of viruses. In addition, full‐length genome analysis could help better trace the spread of canine viruses through different continents.