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Detection and genome sequencing of porcine circovirus 3 in neonatal pigs with congenital tremors in South China
Author(s) -
Chen G. H.,
Mai K. J.,
Zhou L.,
Wu R. T.,
Tang X. Y.,
Wu J. L.,
He L. L.,
Lan T.,
Xie Q. M.,
Sun Y.,
Ma J. Y.
Publication year - 2017
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12702
Subject(s) - porcine circovirus , circovirus , genome , biology , whole genome sequencing , genetics , virology , dna sequencing , medicine , gene , virus
Summary Porcine circovirus 3 ( PCV 3) is a novel circovirus first discovered in the United States in piglets and sows with porcine dermatitis and nephropathy syndrome, reproductive failure, cardiac and multisystemic inflammation. Here, seven PCV 3 strains were identified for the first time from neonatal pigs with clinical signs of congenital tremors ( CT ) in South China. The tissue tropism of PCV 3 in CT ‐affected piglets was analysed by the real‐time quantitative PCR , and the result showed that high loads of viral genomes were detected in the brains and hearts. The complete genomes of seven new PCV 3 revealed 96.8%–99.6% nucleotide identities with eleven other PCV 3 strains previously reported from the United States and China. Phylogenetic analysis based on the complete genome sequences showed that all PCV 3 strains clustered together and were clearly separated from other circovirus species. This study reports on the first identification of PCV 3 in CT ‐affected newborn piglets and provides the epidemiological information of neonatal piglets with CT in Guangdong and Guangxi Provinces of China.