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Novel serotype of bluetongue virus in South America and first report of epizootic haemorrhagic disease virus in Ecuador
Author(s) -
Verdezoto J.,
Breard E.,
Viarouge C.,
Quenault H.,
Lucas P.,
Sailleau C.,
Zientara S.,
Augot D.,
Zapata S.
Publication year - 2018
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12625
Subject(s) - orbivirus , serotype , enzootic , virology , biology , virus , epizootic , reoviridae , serology , antibody , rotavirus , immunology
Summary Bluetongue virus ( BTV ) and Epizootic haemorrhagic disease virus ( EHDV ) are closely related Orbiviruses that affect domestic and wild ruminants. In Ecuador previous serological studies reported the presence of BTV ; however, no data are available about the presence of EHDV . In this study, 295 cattle without symptoms of infection were sampled from two farms located in Andean and Amazonian regions and from a slaughterhouse in the coastal region. ELISA analyses showed high prevalence of BTV (98.9%) and EHDV (81.3%) antibodies, and RT ‐ qPCR s revealed the presence of EHDV (24.1%) and BTV (10.2%) genomes in cattle blood samples. Viral isolation allowed to identify EHDV serotype 1 ( EHDV 1) and BTV serotypes 9 ( BTV 9), 13 and 18. These findings suggest that BTV and EHDV are enzootic diseases in Ecuador.