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Development of a Luminex‐Based DIVA Assay for Serological Detection of African Horse Sickness Virus in Horses
Author(s) -
SánchezMatamoros A.,
NietoPelegrín E.,
Beck C.,
RiveraArroyo B.,
Lecollinet S.,
Sailleau C.,
Zientara S.,
SánchezVizcaíno J. M.
Publication year - 2016
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12503
Subject(s) - diva , african horse sickness , virology , serotype , serology , multiplex , orbivirus , antibody , virus , biology , vaccination , immunology , reoviridae , bioinformatics , rotavirus
Summary African horse sickness ( AHS ) is considered a fatal re‐emergent vector‐borne disease of horses. In the absence of any effective treatment for AHS , vaccination remains the most effective form of disease control. The new generation of vaccines, such as one based on purified, inactivated AHS virus (AHSV, serotype 4), which does not induce antibodies against non‐structural protein 3 ( NS 3), enables the development of diagnostic methods that differentiate infected from vaccinated animals ( DIVA assays). As detecting AHS in AHSV ‐free countries may lead to restrictions on international animal movements and thereby cause significant economic damage, these DIVA assays are crucial for reducing movement restrictions. In this article, we describe a Luminex‐based multiplex assay for DIVA diagnosis of AHS , and we validate it in a duplex format to detect antibodies against structural protein 7 ( VP 7) and NS 3 in serum samples from horses vaccinated with inactivated AHSV 4 vaccine or infected with a live virus of the same serotype. Results of the Luminex‐based assay for detecting anti‐ NS 3 antibodies showed good positive correlation with results from an in‐house enzyme‐linked immunosorbent assay ( ELISA ). Thus, the Luminex‐based technique described here may allow multiplex DIVA antibody detection in a single sample in less than 2 h, and it may prove adaptable for the development of robust, multiplex serological assays.