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Detection and Phylogenetic Analysis of Porcine Deltacoronavirus in Korean Swine Farms, 2015
Author(s) -
Lee J. H.,
Chung H. C.,
Nguyen V. G.,
Moon H. J.,
Kim H. K.,
Park S. J.,
Lee C. H.,
Lee G. E.,
Park B. K.
Publication year - 2016
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12490
Subject(s) - phylogenetic tree , biology , phylogenetic relationship , phylogenetics , genetics , gene
Summary This study applied molecular‐based method to investigate the presence of porcine deltacoronavirus ( PDC oV) in 59 commercial pig farms in South Korea. The results of RT ‐ PCR screening on a relatively large collection of faeces samples ( n = 681) from January 2013 to March 2015 did not reveal the presence of PDC oV until the end of 2014. However, on March 2015, PDC oV‐positive samples ( SL 2, SL 5) were detected from SL swine farm in Gyeongbuk province. The phylogenetic trees based on the complete spike‐ and nucleocapsid protein‐coding genes showed that SL 2 and SL 5 closely related to the US PDC oV strains rather than those in China. Thought Korean strains of PDC oV isolated in 2014 ( KNU 14.04) and in 2015 ( SL 2 and SL 5) grouped within US PDC oV cluster, the reconstruction of ancestral amino acid changes suggested that they are different.